Table 1 Demographic information for cohorts included in the meta-analyses.

From: A meta-analysis of epigenome-wide association studies in Alzheimer’s disease highlights novel differentially methylated loci across cortex

Stage

Cohort

Unique individuals

Ancestry (Eu/Af/Am/As)

Braak

Number

Sex (M/F)

Age at death in (± SD)

Tissues analysed

Discovery

London 1

113

112/0/1/0

0–II

29

13/16

77.6 (12.8)

Prefrontal cortex, entorhinal cortex, superior temporal gyrus, cerebellum (Bulk)

III–IV

18

7/11

88.5 (5.2)

V–VI

66

26/40

85.4 (8.1)

London 2

95

92/1/2/0

0–II

23

12/11

76.1 (10.0)

Entorhinal cortex, cerebellum (Bulk)

III–IV

16

3/13

87.6 (6.4)

V–VI

56

26/30

81.5 (8.6)

Mount Sinai

146

113/20/11/2

0–II

60

32/28

82 (7.6)

Prefrontal cortex, superior temporal gyrus (Bulk)

III–IV

42

12/30

88.8 (6.6)

V–VI

44

12/32

88.0 (7.5)

Arizona 1

302

302/0/0/0

0–II

61

40/21

80.3 (8.2)

Middle temporal gyrus, cerebellum (Bulk)

III–IV

97

50/47

86.9 (6.9)

V–VI

144

63/81

82.3 (8.5)

Arizona 2

88

88/0/0/0

0–II

16

10/6

82.5 (5.0)

Middle temporal gyrus, cerebellum (Bulk)

III–IV

45

21/24

86.7 (5.1)

V–VI

27

12/15

84.6 (7.1)

ROS/MAP

711

711/0/0/0

0–II

143

70/73

83.2 (6.0)

Prefrontal cortex (Bulk)

III–IV

410

144/266

86.9 (4.1)

V–VI

158

45/113

87.8 (3.5)

Replication

Munich

45

–

0–II

9

5/4

76.7 (10.9)

Prefrontal cortex (Bulk)

III–IV

7

1/6

82.1 (5.2)

V–VI

29

12/17

79.2 (8.5)

26

–

0–II

11

7/4

75.9 (8.5)

Occipital cortex (Sorted cells)

III–IV

5

1/4

85.0 (6.5)

V–VI

10

4/6

77.9 (6.6)

BDR

590

–

0–II

196

100/96

83.6 (10.6)

Prefrontal cortex (Bulk)

III–IV

136

80/56

85.1 (7.45)

V–VI

258

128/130

82.5 (8.5)

  1. Sample numbers, split of males (M)/females (F) and mean age at death in years (± standard deviation [SD]) are shown for individuals with low pathology (Braak 0–II), mid-stage pathology (Braak III–IV) and severe pathology (Braak V–VI) in each cohort. Shown are the bulk tissues available from each cohort, which included the cerebellum, entorhinal cortex, middle temporal gyrus, prefrontal cortex and superior temporal gyrus. In the discovery meta-analyses, we used data from six EWAS using the 450K array, which all had >50 unique donors. For replication we used two cohorts. The Munich cohort had 450K data from bulk prefrontal cortex tissue, as well as data available from sorted neuronal and non-neuronal cell populations from the occipital cortex. The BDR cohort had EPIC array data available from bulk prefrontal cortex samples. For the meta-analyses, superior temporal gyrus and middle temporal gyrus samples were both classed as temporal gyrus samples. Shown are final numbers for all cohorts after data quality control. Ancestry is reported for the discovery cohorts and is the number of unique individuals that had the following inferred ethnicities from the 1000 genomes reference panel: European (Eu), African (Af), American (Am), East Asian (As).
Back to article page