Fig. 3: The CASA complex localizes to the sarcomere Z-disc in cardiomyocytes. | Nature Communications

Fig. 3: The CASA complex localizes to the sarcomere Z-disc in cardiomyocytes.

From: Cardiomyocyte contractile impairment in heart failure results from reduced BAG3-mediated sarcomeric protein turnover

Fig. 3

a Immunofluorescence images of human LV cardiomyocytes immunostained for BAG3, HSP70, HSPB8, and CHIP and counterstained for α-actinin; ×63 magnification, scale bars = 5 µm; images are representative 10 images/antibody/5 independent biological samples. b Quantitative line scan of fluorescence intensity by distance from the corresponding green and red channels in panel a. c Western blot for the CASA complex proteins in the whole LV, triton-soluble, and myofilament fractions; sarcomeric α-actin = myofilament fraction positive control, GAPDH = soluble fraction positive control. d Western blot showing results of reciprocal co-immunoprecipitation (IP) for BAG3 and HSP70 in myofilament fraction (IgG—non--specific antibody control); images are representative of three independent experiments. e Western blot showing results of reciprocal co-immunoprecipitation for BAG3 and HSPB8 in myofilament fraction; images are representative of three independent experiments.

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