Fig. 5: BAG3 is required for full assembly of CASA complex members in the myofilament fraction. | Nature Communications

Fig. 5: BAG3 is required for full assembly of CASA complex members in the myofilament fraction.

From: Cardiomyocyte contractile impairment in heart failure results from reduced BAG3-mediated sarcomeric protein turnover

Fig. 5

a Western blots for myofilament BAG3, HSP70, HSPB8, and CHIP in WT, BAG3+/−, and BAG3−/− mice; images are representative of 6 WT hearts, 7 BAG3+/− hearts, and 3 BAG3−/− hearts. b–d Densitometry signal of HSP70 (b), HSPB8 (c), and CHIP (d) normalized to total protein; n = 6 WT, 7 BAG3+/−, 3 BAG3−/−; one-way ANOVA, Tukey post-hoc. e Western blot for myofilament HSP70 in DCM and NF human samples; image is representative of 9 NF hearts and 21 DCM hearts. f HSP70 expression grouped by “high” or “low” BAG3 expression; n = 18 high BAG3, 12 low BAG3. g Western blot for myofilament HSPB8 in NF and DCM human samples; image is representative of 9 NF hearts and 21 DCM hearts. h HSPB8 expression grouped by “high” or “low” BAG3 expression; n = 18 high BAG3, 12 low BAG3. i Western blot for myofilament CHIP in DCM and NF human samples; image is representative of and 9 NF and 17 DCM hearts. j CHIP expression grouped by “high” or “low” BAG3 expression; n = 15 high BAG3, 11 low BAG3 (4 samples not assessed due to lack of remaining tissue). High ≥80% of mean NF expression, Low <80% mean NF expression; two-tailed t-test. All data are presented as mean ± SEM.

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