Fig. 5: PPDPF interacts with Raptor and inhibits the ubiquitination of Raptor.
From: PPDPF alleviates hepatic steatosis through inhibition of mTOR signaling
a, b Co-immunoprecipitation assay in 293T cells co-transfected with Flag-Raptor and HA-PPDPF. c, d Examination of the interaction between endogenous PPDPF and Raptor by co-immunoprecipitation. e PPDPF interacts with Raptor in vitro, purified GST was used as a control. f GST-pull down assay using different truncation mutants of PPDPF. g, h 293 T cells were transfected with control vector and PPDPF WT, PPDPF mut, HA-Raptor plasmid, respectively. After 48 h, co-immunoprecipitation assay was performed. i GST-pull down assay examining the interaction between GST-PPDPF, GST-PPDPF mut with Raptor. j The ubiquitination of Raptor in 293T cells was examined using immunoprecipitation with Raptor antibody. All experiments were repeated 3 times independently.
