Fig. 4: Homothorax (Hth) is a positive regulator of Antennapedia (Antp) in the dorsal and ventral parts of the wing disc pouch.

a Expression of Hth (green) and Antp (red) in the different genetic contexts, as indicated. Yellow arrows highlight the dorsal and ventral part of the pouch where Hth and Antp overlap. Enlargement on co-expressing nuclei (scare) is also indicated on the right. The pouch in wild type or domain of expression of the MS1096 driver is outlined (yellow dotted line). These patterns were systematically observed in dissected discs from two independent experiments (minimum n = 8). The enlargement in the MS1096 > UAS-Hth background is shown from a region of the pouch, where Antp is normally not expressed. b SEM acquisition of wild-type and mutant adult wing, as indicated. The number of wings showing the same phenotype over the total number of dissected wings from adult males (from two independent experiments) is indicated. c Hth-binding profile on the P1 cis-regulatory sequences of Antp from Chip-seq performed in the wing disc, as indicated. Several Hth-binding sites are found in wing-specific chromatin-accessible regions (as deduced from FAIRE analysis and in comparison, to the haltere disc).