Fig. 2: Effect of codon optimization on expression and activity of hG6PC mRNAs. | Nature Communications

Fig. 2: Effect of codon optimization on expression and activity of hG6PC mRNAs.

From: mRNA therapy restores euglycemia and prevents liver tumors in murine model of glycogen storage disease

Fig. 2

a hG6Pase-α protein expression (left panel) and enzymatic activity (right panel) of wild-type hG6PC (WT), codon optimized wild-type hG6PC (WT_CO), hG6PC_S298C (S298C), and codon optimized hG6PC_S298C (S298C_CO) mRNAs evaluated in Hep3B cells. Control cells were treated with eGFP mRNA. Data were presented as mean ± SD of n = 3 biologically independent samples. b hG6Pase-α protein expression (left panel) and enzymatic activity (right panel) of WT and codon optimized hG6PC mRNAs as evaluated in male CD-1 mice. Control animals were treated with eGFP mRNA. Data were presented as mean ± SD of n = 4 mice. For statistical analysis, raw values were Log2 transformed and subjected to one-way ANOVA, followed by the Dunnett’s multiple comparisons test, compared to the non-codon optimized WT hG6PC mRNA. Statistically significant P values (p ≤ 0.05) are shown in the graphs. Source data are provided as a Source Data File.

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