Fig. 2: Xeno-free human EPS cells proliferate well and maintain genome stability after long-term culturing.
From: Chemically defined and xeno-free culture condition for human extended pluripotent stem cells
a Analysis of doubling time of feeder-cultured and xeno-free hEPS cells. n = 3 biologically independent samples. b FACS analysis of the percentages of EDU + cells in feeder-cultured and xeno-free hEPS cells. n = 2 biologically independent samples. c Single-cell cloning efficiency of feeder-cultured and xeno-free hEPS cells. n = 32 biologically independent samples. d Karyotype analysis of xeno-free hEPS cells with different genetic backgrounds. e Chromosomal copy-number analysis of EPS cells by whole-genome sequencing. F-LCDM, hEPS cells cultured on feeders; XF-LCDM, xeno-free hEPS cells. Error bars, mean ± SD. All differences between means with P < 0.01 are indicated. **P < 0.01; ***P < 0.001; ****P < 0.0001. Statistical significance was analyzed using one-way ANOVA with Tukey multiple comparison test (a, c). Experiments in a–c were all independently repeated at least three times with similar results.
