Fig. 5: The xeno-free LCDM condition supports hEPS cell generation from fibroblasts.
From: Chemically defined and xeno-free culture condition for human extended pluripotent stem cells
a A diagram showing the procedures of generating xeno-free hEPS cells from human fibroblasts by reprogramming. b Representative images showing morphological changes of cells during reprogramming. Similar images were obtained in at least two independent experiments. c Representative images showing the morphologies of xeno-free human EPS cells derived from human fibroblasts. Similar images were obtained in at least two independent experiments. d Representative immunostaining images showing pluripotent marker gene expression in xeno-free hEPS cells derived from human fibroblasts. Similar images were obtained in at least three independent experiments. e Representative images showing histological analysis of teratomas generated from xeno-free hEPS cells derived from human fibroblasts. Similar images were obtained in at least three independent experiments. f Karyotype analysis of xeno-free hEPS cells derived from human fibroblasts. g Analysis of global gene expression correlation between feeder-cultured hEPS cells and xeno-free hEPS cells derived from human fibroblasts. F-LCDM, hEPS cells cultured on feeders; XF-LCDM, xeno-free hEPS cells. Scale bars, 100 µm. Experiments in b–e were all independently repeated at least two times with similar results.
