Fig. 5: OSKM induction does not change the self-renewal of SCs.

a Schematic representation of the experimental design for 7 days after triple CTX injury. b Immunostaining of Pax7 and MyoD, and quantification of Pax7 cells in TA muscle sections. Scale bars = 50 µm. Representative regions are shown at higher magnification. Scale bars = 25 µm. White arrows indicate Pax7⁺ cells. c Immunostaining of embryonic myosin heavy chain (eMHC) and Dystrophin in TA muscle sections, and the quantification of the percentage of immature myofibers that express eMHC. Scale bars = 50 µm. d H&E staining of TA muscle sections and myofiber size distributions in TA muscle sections. Scale bars = 100 µm. e Schematic representation of the experimental design for 30 days after triple CTX injury. f Immunostaining of Pax7, BrdU, and MyoD, and quantification of Pax7 cells and percentage of BrdU⁺ SCs in TA muscle sections. Scale bars = 25 µm. Red Arrows indicate Pax7⁺MyoD⁻BrdU⁺SCs. Error bars represent mean + SD of four mice. A two-sided unpaired Student’s t-test was performed.