Fig. 2: Skeletal defects in limb buds lacking three Meis1/2 alleles.
From: Control of mouse limb initiation and antero-posterior patterning by Meis transcription factors
a–f Victoria blue cartilage staining of HLs at E14.5 in WT embryos, with homozygous deletion of Meis2 (M2KO), and M2KO embryos compound with a Meis1 knockout allele in heterozygosity (M1HT;M2KO). Scale bar = 1000 μm. g Percentage size change in skeletal elements of M2KO and M1HT;M2KO HLs. Limbs analyzed: WT N = 6; M2KO N = 4 and M1HM2KO N = 10. ns, non-significant; *p < 0.05, **p < 0.01, ***p < 0.001. Two-tailed, unpaired Mann–Whitney test. Red bars are mean values. Exact p-values from left to right: 0.7619; 0.0420; 0.4121; 0.0001; 0.6485; 0.9623; 0.3152; 0.3148. h Percentage occurrence of skeletal alterations in M1HT;M2KO HLs. Arrowheads in f indicate the absence of fibula and posterior digits in M1HT;M2KO specimens. N = 10 embryos. Source data are provided as a Source Data file. Source images are available at Mendeley Data80 [https://data.mendeley.com/datasets/r774bxyf8d/2].
