Fig. 3: Molecular analysis of Meis function in limb buds.
From: Control of mouse limb initiation and antero-posterior patterning by Meis transcription factors
a RNA-seq analysis comparing wild-type and M1KO;M2KO HLs, showing the clustering of WT and mutant samples by expression profile. b Volcano plot and heat map with the most relevant genes involved in AP patterning, PD patterning, Wnt and Fgf pathways highlighted in color code. p-values are obtained by a Benjamini–Hochberg procedure, which adjusts for multiple comparisons. The underlined highlighted genes show Meis-binding sites in their regulatory regions by ChIP-seq. c–f ChIP-seq analysis, showing the number of binding sites identified in E10.5 FLs and HLs (c), the distribution of Meis peaks with respect to transcriptional units (d) and de novo (e), and known (f) sequence motif identification. FC: fold change. See the “Data availability” section for source data.
