Fig. 1: Zfp541 was identified as a MEIOSIN-STRA8 target gene. | Nature Communications

Fig. 1: Zfp541 was identified as a MEIOSIN-STRA8 target gene.

From: Meiosis-specific ZFP541 repressor complex promotes developmental progression of meiotic prophase towards completion during mouse spermatogenesis

Fig. 1

a Genomic view of MEIOSIN and STRA8 binding peaks over the Zfp541 locus. Genomic coordinates derived from Ensembl. b The expression of Zfp541 in WT (P8 and P10), Meiosin KO, and Stra8 KO testes was examined by RT-qPCR. Three animals for each genotype were used. The graph shows the expression level of Zfp541 normalized by that of GAPDH with SD. The expression level of Zfp541 in P10 WT was set to 1. Statistical significance (two-tailed t-test) is shown by ***p-value = 0.999 × 10−3. Biologically independent mice (N = 3) for each genotype were examined. c The tissue-specific expression pattern of Zfp541 was examined by RT-PCR. Testis RNA was obtained from embryonic day 18 (E18), 3-weeks-old (3w), and 8-weeks-old (8w) male mice. Ovary RNA was obtained from adult 8-weeks-old (8w) female mice. RT− indicates control PCR without reverse transcription. The data were acquired from two separate experiments. d The expression patterns of Zfp541 in the embryonic ovary and testis were examined by RT-qPCR. Average values normalized to E12.5 gonad are shown with SD from technical triplicates. N = 1 gonadal sample for each embryo. e Chromosome spreads of WT spermatocytes were immunostained as indicated. Biologically independent mice (N = 2) were examined in two separate experiments. Scale bar: 5 μm. f Seminiferous tubule sections in WT testis (8-weeks old) were immunostained as indicated. Lep: leptotene, Pa: pachytene, l.P: late pachytene spermatocyte, rS: round spermatid, eS: elongated spermatid. Boundaries of the seminiferous tubules are indicated by white dashed lines. Roman numbers indicate the seminiferous tubule stages. Biologically independent mice (N = 3) were examined in three separate experiments. Scale bar: 25 μm. g Seminiferous tubule sections in WT testis (P21) were immunostained as indicated. Arrow and arrowhead indicate H1t-negative and H1t-positive pachytene spermatocyte, respectively. Biologically independent mice (N = 5) were examined in two separate experiments. Scale bar: 25 μm.

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