Fig. 5: Spermatogenesis was impaired in Kctd19 knockout male.

a The allele with targeted deletion of Exon3-12 in the Kctd19 gene was generated by the introduction of CAS9, the synthetic gRNAs designed to target Exon 3, and the downstream of Exon12 (red arrowheads), and ssODN into C57BL/6 fertilized eggs. Two lines of KO mice were established. Line #12 of Kctd19 KO mice was used in most of the experiments, unless otherwise stated. b Immunoblot analysis of whole testis extracts prepared from mice with the indicated genotypes (P21). Biologically independent mice (N = 2) were examined in two separate experiments. c Seminiferous tubule sections from WT, Kctd19 KO, and Zfp541 KO (8-weeks old) were immunostained as indicated. Biologically independent mice (N = 2) were examined in two separate experiments. Scale bar: 25 μm. d Testes from Kctd19± and Kctd19 KO (8-weeks old). Scale bar: 5 mm. Testis/body-weight ratio (mg/g) of Kctd19± and Kctd19 KO mice (8-weeks old) is shown below (mean with SD). n: number of animals examined. Statistical significance is shown by **p = 0.0012 (two-tailed t-test). e Hematoxylin and eosin staining of the sections from WT, Kctd19±, and Kctd19 KO testes (8-weeks old). Biologically independent mice (N = 3) for each genotype were examined. Scale bar: 50 μm. f Hematoxylin and eosin staining of the sections from WT, Kctd19±, and Kctd19 KO epididymis (8-weeks old). Biologically independent mice (N = 3) for each genotype were examined. Scale bar: 50 μm.