Fig. 3: Patient-derived 3D organoids recapitulate features of parental tumors. | Nature Communications

Fig. 3: Patient-derived 3D organoids recapitulate features of parental tumors.

From: Molecular landscape and subtype-specific therapeutic response of nasopharyngeal carcinoma revealed by integrative pharmacogenomics

Fig. 3

a, b Representative bright field microscopy and H&E staining images of EC-type patient-derived organoids (PDOs) and matched primary tumors from the EC subtype (a); SC-type PDOs and matched primary tumors from the SC and MSEC subtypes (b); c Organoids derived from MSEC subtype tumor (P63) recapitulated the mixed sarcomatoid-epithelial pattern at early passages, later developed two distinct EC-type (PDO63E) and SC-type organoids (PDO63S). d Immunohistochemical staining of AE1/3 on PDOs. EC-type organoids were positive to AE1/3 expression, while organoids derived from MSEC and SC subtypes were AE1/3 negative. e Immunohistochemical staining of vimentin on PDOs. Organoids derived from MSEC and SC subtypes were positive to vimentin expression, while EC-type organoids were vimentin negative. f Immunohistochemical staining of LMP1 on PDOs and parental tumors. PDOs retained EBV infection status. Scale bars (a–f) are 100 μm. The organoid images (a–f) are representatives of 6 EC-type and 6 SC-type organoid lines. g Comparison of the CNV landscape in paired tumors and PDOs. PDOs faithfully preserved chromosomal gains and losses of parental tumors (n = 15 pairs). h Oncoplot comparing the somatic SNVs between PDOs and parental tumors. PDOs maintained important mutations of parental tumors (n = 15 pairs).

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