Fig. 3: Cellular lensing effect.
From: Cellular lensing and near infrared fluorescent nanosensor arrays to enable chemical efflux cytometry
a Instrumental setup for NCC implementation: schematic illustration (left) and a photograph (right). b nIR images of human monocytes flowing (0.5 µL/min) NIM. c Magnified nIR image of single monocyte in NIM (inset: OM image of single monocyte). d FDTD numerical modeling for photonic nanojet and fitting with experimental cellular lensing profile (nc/nm = 1.04, λ = 1 µm). e nIR lensing profiles of a single cell with various focusing points from 5 to 100 µm along Z-stage. nIR lensing effects of f various live cells and g reference micro-particles (top-to-bottom: schematics, OM, nIR images, lensing profiles). h FWHM and i enhancement factors of various cells with numerical model. Data are mean (circle) ± σ (error bar), with ncell = 10. j Schematic illustrations for different lensing behavior of a high RI cell (left) and a low RI cell (right). Scale bars: 20 µm.
