Fig. 3: Field dependence of local values of uncertainty components from axial localization.
From: Accurate localization microscopy by intrinsic aberration calibration
a Line plots showing the relationship z(ρA) for many calibration particles. Three representative lines have colors corresponding to the map in (b) for local values of uncertainty \({\bar{\sigma }}_{z}\) from the polynomial models {z(ρA)}cal. The overbar denotes the mean uncertainty over the axial range of (a). b Scatter plot showing the lateral positions of the calibration particles and corresponding values of \({\bar{\sigma }}_{z}\) for an axial range of 6 µm. c Histogram showing an asymmetric distribution of \({\bar{\sigma }}_{z}\) for an axial range of 6 µm. d Plot showing variation of the mean value of \({\bar{\sigma }}_{z}\) for all particles as a function of axial range for (triangles) {z(ρ)}cal, (squares) {z(ρw)}cal, and (circles) {z(ρA)}cal. e Plot showing variation of the 68 % interpercentile range of \({\bar{\sigma }}_{z}\) for all particles as a function of axial range for (triangles) {z(ρ)}cal, (squares) {z(ρw)}cal, and (circles) {z(ρA)}cal. Roundels in (d–e) correspond to (a–c). Uncertainties in (d–e) are smaller than the data markers.
