Fig. 2: Individual pulses of Msn2 and Msn4 display functional redundancy.

a Example snapshots (top, out of 148, 227, and 262 events, respectively) and schematics (bottom) illustrating three types of TF nuclear localization dynamics (left to right: Msn4-only, Msn2-only, and coincident pulse) and target gene responses (i.e., nascent transcription sites indicated by white arrows). Scale bars indicate 1 μm. b Pulse-triggered averaging analysis¹⁴ of the transcriptional activities of PP7-based synthetic reporter gene (4xSTRE) and endogenous target gene (DDR2) in response to three types of TF dynamics under glucose limitation stress (0.1% glucose). Event numbers are 148, 227, 262, 122, 247, and 260. Error bars indicate 95% CIs and centers indicate means. See also Supplementary Fig. 14a. c Genome-wide characterization of functional redundancy between Msn2 and Msn4 pulses by RNA-seq. Synchronized Msn4-only pulse, Msn2-only pulse, and Msn2/Msn4 co-pulse across cell population as in (a) were achieved by inducing cells of three different genotypes (msn2, msn4, and wild type) by 0.5 M KCl. For each target gene, expression fold change between stress versus non-stress conditions was calculated for each genotype and shown in the ternary plot after normalization. Colormap represents gene density.