Fig. 3: Feeding and electrophysiological responses to low and high concentrations of citric acid for wild-type (wt), otopla1 mutant, and rescue flies.
From: Molecular and cellular basis of acid taste sensation in Drosophila
a Feeding preference between neutral foods (2 mM sucrose) and foods comprising 2 mM sucrose and varying concentrations of citric acid (1 mM, pH 3.7; 10 mM, pH 3.3; 100 mM, pH 2.8; 1000 mM, pH 2.0). Data are presented as mean ± SEM. n = 10 trials, *p = 0.0001 (1 mM) and *p < 0.0001 (10, 50, and 100 mM), one-way ANOVA tests. b Proboscis extension reflex (PER) responses to foods containing 30 mM sucrose and a series of concentrations of citric acid. Data are presented as mean ± SEM. n = 10 trials, *p = 0.0007 (1 mM) and *p < 0.0001 (10, 50, and 100 mM), one-way ANOVA tests. c, d Statistical analyses of spike frequencies evoked by different concentrations of citric acid at the L6 sensilla (c; n = 10 animals, *p < 0.0001 (1–1000 mM), one-way ANOVA tests) and S5 sensilla (d; n = 10 animals). Data are presented as mean ± SEM. e, f Representative spike trains elicited by 10 mM citric acid at the L6 sensilla (e) and S5 sensilla (f). Arrows indicate the onset of taste stimuli.
