Fig. 5: Expression patterns of otopla in the fly taste system.
From: Molecular and cellular basis of acid taste sensation in Drosophila
a, b Immunoreactivity of anti-OtopLa in wild-type (wt; a) and otopla1 (b) proboscises. c Expression of otopla-Gal4 in the labellum. d Distribution of L- and S-type taste sensilla relative to the dendrites of otopla-expressing GRNs in the proboscis. e Double labeling of anti-OtopLa and anti-GFP in otopla-Gal4; UAS-mCD8::GFP flies. f–h Localization pattern of OtopLa in a single GRN dendrite. i–k Localization of otopla-positive sour GRNs relative to Gr5a-positive sweet GRNs (i), Gr66a-positive bitter GRNs (j), and Ir76b-positive salty GRNs (k). l Projection pattern of otopla-positive GRNs in the subesophageal zone (SEZ). Arrow indicates otopla-positive GRN axon bundles. m–o SEZ projections of otopla-positive GRNs relative to Gr5a-positive GRNs (m), Gr66a-positive GRNs (n), and Ir76b-positive GRNs (o). All immunocytochemical experiments were repeated at least three times with similar results. Scale bars: 20 µm (a–d, m–o), 10 µm (e–k), 50 µm (l).
