Fig. 6: Currents evoked by acidic pH in HEK293 cells expressing OtopLa or OtopLaE638A.
From: Molecular and cellular basis of acid taste sensation in Drosophila
a Representative currents produced by the stimuli of acidic isosmotic solutions (pH 3.0) of KCl, LiCl, NaCl, NMDG-Cl, or NMDG-gluconate in HEK293 cells with or without OtopLa expression. Arrows indicate the onset of stimuli. b Statistical analysis of current amplitudes in HEK293 cells with or without OtopLa expression in various bath solutions containing KCl, LiCl, NaCl, NMDG-Cl, or NMDG-gluconate. n = 11 cells, *p < 0.0001 for all bath solutions, unpaired two-tailed t-tests. One-way ANOVA tests comparing current amplitudes of OtopLa-expressing cells in KCl, LiCl, NaCl, NMDG-Cl, and NMDG-gluconate bath solutions gave p = 0.95, indicating no significant differences among these five bath solutions. In the box and whisker plot, top and bottom whiskers represent the maximum and the minimum, respectively; top and bottom box boundaries represent third and first quartiles of the data, respectively; and the center line represents the median. c, d Currents elicited by acidic isosmotic solutions (pH 3.0–6.0) in HEK293 cells expressing OtopLa or OtopLaE638A, and controls. NMDG-gluconate as bath solution. Arrows in c indicate the onset of stimuli. Data are presented as mean ± SEM. n = 12 cells, *p < 0.0001 (pH 3.0–6.0), one-way ANOVA tests.
