Fig. 3: Single-molecule multiplexed detection using synthetic miRNAs.

a–d Intensities-time traces (i) and representative events (ii) for the translocation of a Probe-375 (10 kbp) and Probe-141 (38.5 kbp) on their own, b Probe-375 and Probe-141 in the presence of miR-375-p, c Probe-375 and Probe-141 in the presence of miR-141-3p, and d Probe-375 and Probe-141 in the presence of both miR-375-3p and miR-141-3p. Scale bar in (i): photon trace (green), vertical, 100 counts/ms, horizontal, 3 s; current trace, vertical, 30 pA, horizontal, 3 s. Scale bar in (ii): photon trace (green), vertical, 100 counts/ms, horizontal, 5 ms; current trace, vertical, 25 pA, horizontal, 5 ms. e Histograms summarising the ratio of synchronised events based on the data obtained from a–d. f Histograms for the synchronisation ratio for 3 miRNA populations. All DNA carriers and miRNAs were used at a concentration of 10 pM. The translocations were performed in 100 mM KCl buffer (5 mM MgCl₂, 10 mM Tris-HCl, 1 mM EDTA, pH = 8.0) at an applied potential bias of −300 mV. Laser power was 90 ± 4 µW. The error bars in e and f represent the standard deviation measured from 3 different nanopore sensors (n = 3). Data are presented as mean ± s.d.