Fig. 1: Decreased binding of BRD4 at CREs in Y430C mESCs.
From: Cornelia de Lange syndrome-associated mutations cause a DNA damage signalling and repair defect
a Cartoon of BRD4 showing location of the Y430C mutation in the second bromodomain (BD2). b Heatmaps show enrichment of wild-type (WT) and Y430C BRD4 ChIP over super enhancers (SE), typical enhancers, promoters and gene bodies. c UCSC genome browser screenshot showing reads per 10 million over the Klf4, extended Nanog and Sox2 loci for BRD4 ChIP-seq in WT and Y430C mESCs. Extent of SEs are shown in blue. Below are shown previously published ChIP-seq data for H3K27ac (ENCSR000CDE), H3K9ac (ENCSR000CGS), H3K122ac (GSE66023) and DNase I hypersensitivity (DHS). Genome co-ordinates (Mb) are from the mm9 assembly of the mouse genome. Biological replicate from an independent Y430C clone are in Supplementary Fig. 1. d ChIP-qPCR measuring concentration of BRD4 ChIP DNA relative to input across the SEs of Oct4, Klf4, Nanog, and Sox2; in WT and Y430C mESCs. Data are represented as mean ± SEM from 3 technical replicates.
