Fig. 3: Increased G2/M checkpoint activation in Y430C mESCs.
From: Cornelia de Lange syndrome-associated mutations cause a DNA damage signalling and repair defect
a Graph shows average number of WT and Y430C cells per well at 1, 2, 3 and 4 days post seeding. Data are represented as mean ± SEM from 3 technical replicates. b Overlaid graphs show WT and Y430C cell cycle profiles, as determined by flow cytometry. Graphs illustrate the cell count, which correlates to propidium iodide intensity. Biological replicate in Supplementary Fig. 4a. c Immunoblot using antibodies against Lamin B CHK1 and CHK1-P after treatment of WT and Y430C mESCs with NCS and for various times (hrs) of recovery (source data are provided as a Source Data file). Data from an independent experiment are presented in Supplementary Fig. 4.
