Fig. 4: Increased size and number of 53BP1 foci after DSB induction in Y430C mESCs.
From: Cornelia de Lange syndrome-associated mutations cause a DNA damage signalling and repair defect
a Immunofluorescence for 53BP1 in the DAPI-stained nuclei of wild-type and Y430C mESCs upon treatment with NCS and after recovery periods up to 20 h. Scale bars: 2 μm. The experiment was repeated 3 times independently with similar results. b, c Box-plots show area (μm2) and number of 53BP1 foci per cell, respectively, in WT and Y430C cells after treatment with NCS in one representative experiment. Horizontal lines within boxes show medians, boxes are inter-quartile ranges and whiskers are range. P-values were calculated with Mann–Whitney one-sided U test. *<0.05, **<0.01, ***<0.001. 50 cells per condition were examined. Data from three independent experiment are in Supplementary Fig. 6.
