Fig. 5: Depletion of STBD1 alters multiple genes critical for glycolysis.
From: Model-based analysis uncovers mutations altering autophagy selectivity in human cancer
a Three biological replicates of shControl and shSTBD1 HCT116 cells were clustered together in RNA-seq. b Heatmap of differentially expressed genes in shControl and shSTBD1 HCT116 cells. c The numbers of down-regulated or up-regulated genes in differentially expressed genes. d The KEGG-based enrichment analysis of biological pathway of differentially expressed genes. e mRNA levels of several genes responsible for glycolysis were determined by RT-PCR and normalized using TUBB mRNA. These relative mRNA levels in shSTBD1 vs. shControl HCT116 cells, shSTBD1/W203C vs. shSTBD1/WT cells, and shSTBD1/WT vs. shSTBD1/plvx neo cells were shown. f Enrichment analysis of cancer hallmark traits affected by STBD1 depletion. Representative hallmark genes are shown in the circle. g Protein levels of STBD1, AKT1, c-Myc, and NFKB1 (p50) in shControl and shSTBD1 HCT116 cells were determined by immunoblotting and normalized using Tubulin or GAPDH. Experiments in e, g were performed in triplicate. Statistical data are presented as mean ± SD. Statistical comparisons were performed using an unpaired t test. ***p < 0.001, **p < 0.01, *p < 0.05.
