Fig. 2: Serum metabolites associated with fasting and sirtuin overexpression.
From: Restoration of energy homeostasis by SIRT6 extends healthy lifespan
a Heatmap showing serum metabolites from key metabolic pathways that changed significantly either with dietary state (fed, and 4 h and 16 h of fasting), genotype, or both as measured in two-way ANOVA. Each square represents average metabolite abundance of 7 WT mice, 7 SIRT1-tg mice, 6 SIRT6-tg mice, and 5 SIRT1 + 6-tg mice. b–d Scatterplots of data from (a) showing the levels of BCAAs (b), glucose and the GNG precursors glycerol and lactate (c), and TCA cycle metabolites (d). For (b–d), n = 7 WT mice, n = 7 SIRT1-tg mice, n = 6 SIRT6-tg mice, and n = 5 SIRT1 + 6-tg mice. Box extends from the 25th to 75th percentiles, line in the middle of the box is the median and whiskers go down to the smallest value and up to the largest. Statistical analysis was performed using two-way ANOVA (time, genotype) with Dunnett’s post hoc correction. p-value for genotype or fasting effect is indicated in graphs, exact p-values are reported in Supplementary Table 4. *p < 0.05, **p < 0.01.
