Fig. 1: SUN1 directly interacts with SPDYA.
From: The SUN1-SPDYA interaction plays an essential role in meiosis prophase I
a Yeast two-hybrid (Y2H) interaction analysis of the interaction between N-terminus of SUN1 (residues: 1–210) and the indicated proteins. Interactions between LexA-SUN1 and N-terminally GAD-fused indicated proteins were analyzed by measuring the β-galactosidase activity produced by the reporter gene. Vector, vector only. Data are presented as the mean ± SD for n = 3 independent experiments. b Domain organization of SUN1, SPDYA, and CDK2. The shaded areas indicate the interactions among these proteins. The interacting domains are labeled and highlighted in different colors. ERD extended Ringo domain, SBM SPDYA-binding motif, TM transmembrane, CC coiled-coil domains. c Ribbon diagrams of two orthogonal views of the SUN1SBM–SPDYAERD–CDK2 ternary complex. SUN1 SBM, SPDYAERD, and CDK2 are colored in yellow, blue, and green, respectively. d Superimposed structures of the SUN1SBM–SPDYAERD–CDK2 ternary complex and the binary SPDYARingo–CDK2 complex21. SUN1SBM, SPDYAERD, and CDK2 of the ternary complex are displayed in yellow, cyan, and green, respectively. SPDYARingo and CDK2 of the binary complex are displayed in magenta and blue, respectively. e SUN1SBM forms an antiparallel β sheet with β1 of SPDYAEXD. Residues important for the interaction are shown as stick models. f Detailed interactions at the interfaces between SUN1 SBM and SPDYAERD. g Co-IP measurement of ectopically expressed human Flag-SUN11-210, Myc-SPDYA, and CDK2 in HEK293T cells. The immunoprecipitation is performed with anti-Flag beads. The levels of each protein in the input and IP samples were analyzed by immunoblotting with the indicated antibodies. Band intensity of blots was quantified by the ImageJ software (NIH, https://imagej.nih.gov/ij/) and the co-IP signal for the sample with expression of WT Flag-SUN11-210 and Myc-SPDYA was set as 1. Source data are provided as a Source Data file.
