Fig. 4: The SUN1–SPDYA interaction regulates telomere–LINC complex connection and telomere NE attachment.
From: The SUN1-SPDYA interaction plays an essential role in meiosis prophase I
a IF staining of spermatocyte chromosome spreads for SYCP3 (green) and SUN1 (red). DNA was stained by DAPI (blue). Scale bar, 10 μm. b IF-FISH staining of spermatocyte chromosome spreads for SYCP3 (green), SPDYA (red), and telomeric DNA (TelC, magenta). (Zyg zygotene, Pac-like pachytene-like). Scale bar, 10 μm. c Quantification of the relative intensity of SPDYA foci at telomeres in panel b. A total of n = 8 WT Zygotene spreads (557 telomeres) (WT-Z), n = 12 WT pachytene spreads (498 telomeres) (WT-P), n = 19 W151R zygotene spreads (1269 telomeres) (W151R-Z) and n = 15 W151R pachytene-like spreads (733 telomeres) (W151R-Plike) were analyzed. Red lines indicated the mean values. d Equator images of structurally preserved zygotene spermatocytes stained for SYCP3 (green), Lamin B1 (red), and telomere FISH (TelC, magenta). Scale bar, 5 μm. e Quantification of the number of telomere foci at the nuclear periphery (peri) or internal domain (int) as shown in panel d. A total of n = 20 WT and n = 28 W151R spermatocytes were counted. Red lines indicated the mean values. A two-sided Student’s t test was performed, ***P = 2.6E-10. Source data are provided as a Source Data file.
