Fig. 4: FACS analysis of OSCAR in vivo.
From: Establishment of a fluorescent reporter of RNA-polymerase II activity to identify dormant cells
a FACS analysis of EF1-OSCAR mouse small intestinal EpCAM+ live cells shows several populations of OSCARhigh (VeN90high mCherrylow) and OSCARlow (VeN90low mCherryhigh). In addition, populations with a higher or lower general transgene expression can be detected. b FACS gating strategy used for FACS sorting of populations P1 to P5. c Population P2 contains OSCARhigh cells. Populations P1 and P3 contain OSCARlow cells, with P3 having higher VeN90 and mCherry fluorescence. Populations P4 and P5 are OSCARmed, with P5 having higher VeN90 and mCherry fluorescence. n = 4 mice were analyzed. Data are presented as mean value ± SD. d Percentage of cells in each of the indicated gates with respect to their parental gate. n = 4 mice were analyzed. Data are presented as mean value ± SD. e Hierarchical clustering and heatmap of the Pearson correlation of the RNAseq datasets from the different OSCAR cell populations. f Principal component analysis (PCA) of the RNAseq datasets as in (e). Source data are provided as a Source Data file.
