Fig. 5: The intestinal type 2 immune response induced by parasitic infections and succinate is impaired in Lrmp−/− mice.

a–f WT and Lrmp−/− mice were infected with Tm or Nb and examined at 21 d.p.i. (for Tm) and 5 or 7 d.p.i. (for Nb), respectively. a and b Quantifications of tuft (a) and goblet (b) cells in the small intestine of mice by IHC staining of Dclk1 and Alcian blue staining, respectively. c and d Quantifications of eosinophils (c) and ILC2s (d) in the LP by flow cytometric analysis. e Relative IL-13 mRNA levels in the small intestine determined by quantitative real-time PCR assays. f Egg counts in feces (left) and worm burden (right) in the intestine in Nb-infected mice. g–i The impaired type 2 immune response induced by succinate (150 mM for 7 days) in Lmrp−/− mice. g Quantifications of tuft and goblet cells in the small intestine. h Quantifications of eosinophils and ILC-2s in the LP. i Relative IL-13 mRNA levels in the small intestine. Data are presented as mean ± SEM. Each dot represents an individual mouse. n = 5–8/group. *p < 0.05; **p < 0.01; ***p < 0.001; NS: non-significant, two-tailed Student’s t-test.