Fig. 2: CG11070, a miR-9a target identified from secondary screening, strongly modulated hTau in Drosophila eyes.

a Screening of flies with RNAi knockdown of miR-9a targets in GMR > hTau Drosophila eyes identified a significant reduction in eye size in CG11070-RNAi flies relative to the control. b Volcano plot of mean eye sizes of flies expressing various miR-9a target gene RNAis in GMR > hTau flies versus their respective p-values derived from a one-way analysis of variance followed by pairwise t-tests and a Bonferroni correction for multiple comparisons. All points above the dotted line can be considered significant. CG11070-RNAi flies exhibited more severe ocular Tau toxicity, as demonstrated by decreased eye size. N = 3 biologically independent experiments. c, d The knockdown of CG11070 (CG11070-RNAi) in GMR > hTau flies significantly decreased eye size relative to GMR > hTau controls. N = 5 biologically independent experiments. In the box plots the whiskers represent the 5th to 95th percentile range. Data are presented as the mean ± s.e.m. Statistical significance was determined with a two-tailed Student’s t-test. e miRNA–mRNA–RISC pull-down assays in Drosophila S2 cells revealed that miR-9a bound to CG11070 mRNA. Transfection of miR-9a enriched CG11070 mRNA levels, similar to the known miR-9a targets senseless and sNPFR1, as demonstrated by qRT-PCR. N = 4 biologically independent experiments. Data are presented as the mean ± s.e.m. Statistical significance was determined with a two-tailed Student’s t-test. f Expression of miR-9a using GMR-GAL4 significantly decreased CG11070 expression. N = 3 biologically independent experiments. Data are presented as the mean ± s.e.m. Statistical significance was determined with a two-tailed Student’s t-test. Statistical source data.