Fig. 6: Tau oligomers were degraded by UBE4B and STUB1 in the Tau-BiFC mouse model.
From: UBE4B, a microRNA-9 target gene, promotes autophagy-mediated Tau degradation
a Schematic representation of AAV-CMV-mCherry and AAV-UBE4B-mCherry virus constructs. b Schematic illustration of AAV-CMV-mCherry or AAV-UBE4B-mCherry virus delivery into the dentate gyrus of Tau-BiFC mice. c A fluorescence staining image indicating the foci (red) of AAV-CMV-mCherry or AAV-UBE4B + STUB1-mCherry virus delivery in the dentate gyrus and hippocampus of Tau-BiFC mice. Scale bar (white), 1 mm. d Schematic illustration of the work flow for virus injection and pathological examination in Tau-BiFC mice. e AAV-UBE4B and AAV-STUB1 decreased oligomer Tau-BiFC and pTau (S202/T205) levels in the dentate gyrus compared with the control. GCL, granular cell layer; POL, polymorphic layer. Scale bars (white), 80 μm. These experiments were performed four times. f, g Densitometry analysis revealed that AAV-UBE4B and STUB1 significantly decreased both Tau-BiFC and pTau (S202/T205) levels in the dentate gyrus relative to the control (AAV-Cont N = 4; AAV-UBE4B N = 4; AAV-STUB1 N = 4; AAV-UBE4B + AAV-STUB1, N = 4; N = 4 biologically independent animals), respectively. In the box plots the whiskers represent the 5th to 95th percentile range. Data are presented as means as ± s.e.m. Statistical significance was determined with two-tailed Student’s t-test. Statistical source data.
