Fig. 2: MTERF4–NSUN4 and MRM2 interaction with the mt-LSU assembly intermediates.
From: Structural basis for late maturation steps of the human mitoribosomal large subunit
a Comparison of the MTERF4–NSUN4 complex bound to the GTPBP5KO/IP mt-LSU (orange and green, respectively) with the MTERF4–NSUN4 crystal structure (PDB: 4FP96) (yellow and brown, respectively), and of uL2m from the GTPBP5KO/IP mt-LSU (pink) with uL2m from the mature mt-LSU (blue) (PDB: 3J7Y23). The uL2m C-terminus is indicated in both structures. Pre-H68-71 is not shown. b MTERF4–NSUN4 complex bound to pre-H68-71. Zoom-in panels show the interactions of MTERF4 with the pre-H68-71. c Schematic representation of MRM2 domains (NTD—light pink, SAM MTase domain—red). d MRM2 interaction with the domain IV rRNA (nucleotides 2644–2652, green) and the A-loop (grey). The MRM2 methylation site (U3039), as well as the catalytic triad of MRM2 (K59, D154, and K194), are highlighted as sticks. e Zoom-in views showing MRM2 interactions with the A-loop in different orientations.
