Fig. 6: Functional motif of Nron interacts with Cul4b to regulate ERα ubiquitination.
From: A functional motif of long noncoding RNA Nron against osteoporosis
a Genome browser depiction of Nron and its conserved analogs in multiple species. b Evolutional tree of NCM1 and NCM2 in multiple species. c Expression of ERα in osteoclasts with Nron, NCM1, or NCM2 overexpression analyzed by WB. The experiment was repeated three times independently with similar results. d Expression of ERα in human THP-1 derived osteoclast with NRON, NCM1(h), NCM2(h) overexpression were analyzed by WB. The experiment was repeated three times independently with similar results. e Schematic diagram illustrating the mutation strategies of Nron. f Interactions of Nron and Nron mutants with CUL4B determined by RNA pull-down assay in mouse osteoclast lysates. The experiment was repeated three times independently with similar results. g The ubiquitination of ERα in Nron or NCM2 overexpression osteoclasts were assessed by immunoprecipitation (IP) with anti-ERα antibody followed by ubiquitin WB analysis. The experiment was repeated three times independently with similar results. Source data are provided as a Source Data file.
