Fig. 8: Functional motif of Nron improves cortical bone strength in OVX mice.

a Schematic diagram illustrating the experimental design of NCM2 treatment in OVX mice. b Body weight of the mice from Sham, OVX, OVX-Vector, and OVX-NCM2 group, (n = 6/group). c Spleen index (spleen weight (mg) divided by body weight (g)) of mice from Sham, OVX, OVX-Vector, and OVX-NCM2 group, (n = 6/group). d Representative μCT images showing the 3D cortical bone structures of mice femurs from Sham, OVX, OVX-Vector, and OVX-NCM2 group, (similar results were obtained in all mice, n = 6/group). Scale bar: 1 mm. e μCT measurements of Ct.Th in femurs from Sham, OVX, OVX-Vector, and OVX-NCM2 group, (n = 6/group). f Representative cortical bone H&E staining images of femurs from Sham, OVX, OVX-Vector, and OVX-NCM2 group, (similar results were obtained in all mice, n = 6/group). Scale bar: 100 μm. g Biomechanical analysis of femur from mice as in d, (n = 6/group). The parameters measured include the ultimate force (N), stiffness (N/mm), maximum energy absorption (N·mm). h Representative TRAP staining images of the femur cortical from Sham, OVX, OVX-Vector, and OVX-NCM2 group, (similar results were obtained in all mice, n = 6/group). Scale bar: 100 μm. i Quantification of the endocortical surface osteoclastic metrics Oc.N/B.Pm and Oc.S/BS in (h), (n = 6/group). Data are presented as the means ± s.e.m. The significant differences between Sham/OVX-Vector/OVX-NCM2 treatment group with OVX group were determined by one-way ANOVA with Dunnett’s multiple comparisons test. Source data are provided as a Source Data file.