Fig. 2: Islets from HFD beta-barr1-KO mice show deficits in beta-cell mass, size, and proliferation rate, and altered expression of key beta-cell genes.
From: β-Arrestin-1 is required for adaptive β-cell mass expansion during obesity
a–c Beta-cell mass (a), islet size (b), and beta-cell size (c) are significantly decreased in HFD beta-barr1-KO mice, as compared to HFD control mice. d Number of islets per area of pancreas section (arbitrary units). e Rate of beta-cell proliferation (Ki67/insulin co-staining experiments). f Expression levels of genes involved in the maintenance and function of beta-cells, as studied by qRT-PCR. RNA was isolated from pancreatic islet from HFD control and beta-barr1-KO mice. Data were given as means ± s.e.m. (a, 8 control and 9 KO mice, respectively; b, 9 control and 15 KO mice, respectively; c, 75 and 73 cells, respectively, were examined from three mice per group; d, 9 control and 15 KO pancreatic sections were examined from three mice per group; e, data were from four mice per genotype; f, data were from at least four mice per genotype). P values are indicated in the different panels (unpaired two-tailed t-test). Source data are provided as a Source data file.
