Fig. 2: CAB drug plasma concentrations in Balb/c and NSG mice.

Male Balb/c or female NSG mice were given a single intramuscular injection of 45 or 70 mg CAB equivalents/kg of NCAB or NM2CAB. Plasma was collected every other week for 6 months at which time the study was terminated. CAB concentrations were quantitated by LC-MS/MS by Covance laboratories (a–d); treatment with 45 mg/kg in male Balb/c (a) and female NSG mice (b); treatment with 70 mg/kg in male Balb/c (c) and female NSG mice (d). CAB concentrations in plasma samples were also quantitated at UNMC (bioanalytical confirmation). Plasma CAB concentrations for the 45 mg/kg treatment groups are shown for male Balb/c (e) and female NSG mice (f). The dotted line indicates the CAB protein-adjusted IC₉₀ (PA-IC₉₀). Data are expressed as mean ± SEM (N varied from 2 to 6 animals per group per time point due to loss of animals by natural causes, in both NCAB and NM2CAB treatment groups, or inadequate plasma volume collected during the study period, with exact values for each time point provided in the source data [10.6084/m9.figshare.14498025]).