Fig. 1: Regulation of l-2-HG catabolism by LhgR in P. putida W619.
From: An l-2-hydroxyglutarate biosensor based on specific transcriptional regulator LhgR
a Schematic representation of genomic neighborhood analysis of lhgO in different bacteria. Orthologs are shown in the same color and the direction of gene transcription is indicated by arrows. CsiR, GntR family allosteric transcription factor regulating glutarate catabolism; CsiD, glutarate hydroxylase; LhgO, l-2-HG oxidase; GabD, succinate semialdehyde dehydrogenase; GabT, 4-aminobutyrate aminotransferase; GabP, 4-aminobutyrate transporter. b, c Growth of the derivatives of P. putida KT2440 in MSMs with glutarate (b) or l-2-HG (c) as the sole carbon source. Growth (closed symbols) and the consumption of carbon source (open symbols) of P. putida KT2440 (ΔlhgO) harboring plasmid pME6032-lhgO (black lines with squares) and P. putida KT2440 (ΔlhgO) harboring empty plasmid pME6032 (red lines with circles) were measured in MSMs containing 5 g L−1 glutarate (b) or l-2-HG (c) as the sole carbon source. d Schematic representation of the construction of pME6032-F2-lhgR-F1-lhgO and its transfer into P. putida KT2440 (ΔcsiRΔlhgO) and P. putida KT2440 (ΔcsiRΔcsiDΔlhgO) by electroporation. The deleted genes in P. putida KT2440 are indicated by dashed lines. The reaction catalyzed by CsiD is also demonstrated. e The activities of LhgO in P. putida KT2440 (ΔcsiRΔlhgO) and P. putida KT2440 (ΔcsiRΔcsiDΔlhgO) harboring either plasmid pME6032-F2-lhgR-F1-lhgO or empty plasmid pME6032 grown in MSM with glutarate, l-2-HG, or d-2-HG as the sole carbon source. All data shown are means ± standard deviations (s.d.) (n = 3 independent experiments).
