Fig. 4: Validation of purified LHGFR for determination l-2-HG levels in body fluids and bacterial culture system.
From: An l-2-hydroxyglutarate biosensor based on specific transcriptional regulator LhgR
a–d Dose-response curves of purified LHGFR0N3C and LHGFR0N7C for increasing concentrations (10 nM to 2 mM) of l-2-HG in serum (a, b) and urine (c, d). e, f Comparison between the quantitative results of l-2-HG added in serum (e) and urine (f) by LC-MS/MS and LHGFR. The gray circles and red circles represent the quantitative results of LHGFR0N3C and LHGFR0N7C, respectively. Black line indicates a reference line. g, h Dose-response curves of purified LHGFR0N3C (g) and LHGFR0N7C (h) for increasing concentrations (10 nM to 2 mM) of l-2-HG in bacterial culture medium. i Comparison between the quantitative results of l-2-HG in bacterial culture medium by LC-MS/MS and LHGFR. j Growth of P. putida KT2440 and its lhgO mutant in medium containing 20 mM glucose and 5 mM glutarate as the carbon sources. k, l Determination of extracellular l-2-HG accumulation of P. putida KT2440 and its lhgO mutant by purified LHGFR0N3C (k) and LHGFR0N7C (l). All data shown are means ± s.d. (n = 3 independent experiments).
