Fig. 3: Reverse-translocation dynamics.

a An individual reverse-translocation event is divided into three sections to differentiate the first and last dwell from the dwells during burst-sized slipping (slipping dwells). b Violin plots and bar graphs of select aspects of the reverse-translocation events on dsRNA under different [ATP] and opposing force are shown. Data are from n = 57, 85, and 83 reverse-translocation events for 0.025 mM [ATP] 10 pN, 0.25 mM [ATP] 10 pN, and 0.25 mM [ATP] 30 pN, respectively. c Violin plots and bar graphs showing the differences of select statistics when the substrate is the RTS hybrid compared to dsRNA. Data are from n = 21, 85 reverse-translocation events for RTS hybrid and dsRNA, respectively. As the total length is equal to the slipping velocity times, the mid-time, the trend in total length is identical to that of the mid-time graph, as the slipping velocities for RTS hybrid and dsRNA are the same. [ATP] is 0.25 mM and the opposing force is 10 pN. For b and c, horizontal black lines on the violins are the median of the corresponding distribution and errors on bar graphs are SEM.