Fig. 2: Structures of two NTE segments of RRFmt.
From: Distinct mechanisms of the human mitoribosome recycling and antibiotic resistance
a Density corresponding to the N-terminus segment of RRFmt NTE (pink) observed in the inter-subunit space sandwiched between the 28S SSU components, including MRP uS12m (cyan) and the 12S rRNA helix h44 (brown), the 39S LSU components, including 16S rRNA helices H69 (medium blue) and H71 (dark blue), and the outer bent of the junction between domains I and II of RRFmt. The dotted line (pink) depicts the connection between two structurally stabilized segments of the RRFmt’s NTE. b H69 superimposition from bacterial (gray)33 and human mitochondrial ribosomes (blue) reveals the shortening of H69 in the mitoribosome, also depicted in secondary structures of H69 on the lower left. Domain II of RRFmt interacts with the shorter H69 through its strategically positioned N-terminus of NTE (pink). c Sequence of NTE showing two modeled regions (pink). Thumbnails to the left depicts an overall orientation of the 55S mitoribosome, with semitransparent 28S (yellow) and 39S (blue) subunits, and overlaid positions of ligands. Landmarks on the thumbnail: h, head, and b, body of the 28S subunit, and CP central protuberance, Sb stalk base of the 39S subunit.
