Fig. 1: Rust response phenotypes conferred by the Sr26 and Sr61 parental sources and mutant derivatives, and gene structures of Sr26 and Sr61 candidate genes.
From: A recombined Sr26 and Sr61 disease resistance gene stack in wheat encodes unrelated NLR genes
a Abaxial seedling leaf surfaces of wild-type and representative EMS-generated susceptible mutants for Sr26 and Sr61, together with recombinant 376/15 inoculated with Pgt race PTKST. Avocet, Kite, Avocet+Lr46, W3757, and recombinant 376/15 showed low infection types (small pustules or flecking), whereas Sr26 mutant 12S, Sr61 mutant M4 and susceptible control line 37-07 all showed high infection types (large pustules). Bar shows 1 cm. b Gene structures of candidate Sr26 and Sr61 genes. Exons are shown as solid boxes with the terminal exon indicated as an arrow to show the direction of transcription whereas introns are shown as solid black angled lines. The position of mutations in mutant lines are shown in red with predicted amino acid substitutions caused by nonsense mutations and premature stop codon shown above. CC domain, NB domain, and LRR motifs are shaded upon the exons with yellow, blue, and green colors.
