Fig. 2: Validation of the Sr26 and Sr61 candidate genes by transformation.
From: A recombined Sr26 and Sr61 disease resistance gene stack in wheat encodes unrelated NLR genes
a Four constructs used for wheat transformation. The three Sr26 constructs encoded the Sr26 gene candidate and either its native regulatory sequences (Sr26:NativeRE), regulatory sequences from Sr22 (Sr26:Sr22RE) or regulatory sequences from Sr33 (Sr26:Sr33RE). A single construct was used for the Sr61 gene candidate under the regulatory control of Sr26 5′ and 3′ regulatory elements (REs). 5′ REs and 3′ REs are indicated by black brackets with sizes in bp indicated for all four constructs. Sr26 and Sr61 intron/exon coding regions are shown as light and dark blue arrows. b Phenotypic responses of representative T0 plants produced for all four constructs and inoculated with Pgt race 98-1,2,3,5,6+Sr50 along with non-transgenic Fielder control lines. All lines except the susceptible Fielder control showed low infection types. Photographed 12 days of post-inoculation. Bar shows 1 cm.
