Fig. 1: The frequency of 5-FC resistance varies according to 5-FC concentrations.
From: Moderate levels of 5-fluorocytosine cause the emergence of high frequency resistance in cryptococci
Cells from H99, R265, and WM276 were plated on YNB media supplemented with indicated amounts of 5-FC which were approximately corresponding to 20-, 40-, 100-, and 400-fold of 5-FC MIC of each tested strain. Plates were incubated at 30 °C for 7 days and photographed. The frequency of 5-FC resistance was calculated by dividing the number of the 5-FC resistant colonies by the total number of the input cells. The experiments were repeated three times.
