Fig. 6: The Ugd1 function or the UGD1 expression levels is affected in suppressor containing strains.
From: Moderate levels of 5-fluorocytosine cause the emergence of high frequency resistance in cryptococci
a–c Capsule size is affected by the UGD1G19A mutation. Cells were stained by India ink and visualized by microscopy. The experiments were repeated 4 times independently with similar results. The capsule sizes of more than 65 cells were measured from 5 different fields for each indicated strain. Capsular thickness was defined as the distance between the visualized cell wall and the edge of the capsule. The box-and-whiskers plot is used to show median, quartiles (boxes), and range (whiskers). Two-tailed, unpaired t-test (****p < 0.0001). Bar = 5 μm. d UGD1 expression is downregulated in the Knrg1 containing strains. RNA was isolated and the expression levels of UGD1 were determined by qRT-PCR, normalized to the actin gene. The experiments were repeated three times. One-way ANOVA with Tukey’s test for multiple comparisons (ns = not significant and ****p < 0.0001).
