Fig. 7: The accumulation of [3H] 5-FC is low in the uxs1 mutants and high in the suppressor strains.
From: Moderate levels of 5-fluorocytosine cause the emergence of high frequency resistance in cryptococci
Cells were incubated with [3H]-flucytosine and the accumulation of [3H]-flucytosine was determined at indicated time. The experiments were repeated three times and data are presented as mean values ± standard deviation. Each panel contains the wild type, the uxs1 mutant and the suppressor strains derived from WM276 (a), WL8 (b), WL9 (c) and KN99α (d). Two-way ANOVA with Tukey’s multiple comparisons test was used to compare the differences at the indicated time point between the uxs1 mutant and the wild type or between the uxs1 mutant and the suppressor strain (ns = not significant, **p < 0.0021, ***p < 0.0002, and ****p < 0.0001).
