Fig. 1: H3K27me3 demethylase activity is required for acquired thermotolerance.
a Schematic representation of the temperature conditions used in this study. The time of phenotyping is indicated with a green dot. Left, normal plant growth conditions. Center, basal thermotolerance conditions (+HS). Right, heat-stress memory conditions (+ACC +HS). b, c Wild type (b) and jmj30 jmj32 ref6 elf6 quadruple mutants (jmjq) (c) grown under control conditions. d, e Wild type (d) and jmjq (e) grown under +HS conditions. f, g Wild type (f) and jmjq (g) grown under +ACC +HS conditions. Insets show scanning electron microscope images of representative shoot apical meristems. h Quantification of survival rate. In all, 10-day-old seedlings grown under the three different temperature conditions were categorized into three groups based on phenotypic severity: green, normal growth; light green, partially damaged; and white, perished. Different letters indicate significant differences. Significance was determined by the χ2 test followed by a post-hoc test. n > 138. i Quantification of chlorophyll contents. Sample minimum (lower bar); lower quartile (box); median (middle line); upper quartile (box); and sample maximum (upper bar). Light green dots and white circles represent the chlorophyll contents of individual samples and statistical outliers, respectively. One-way ANOVA test, *p < 1.0 × 10–3; **p < 1.0 × 10–4. Different letters indicate significant differences based on post-hoc Tukey’s HSD test. p-values based on post-hoc Tukey’s HSD test between wild type and jmjq mutants are shown. p < 0.05. NS nonsignificant. n = 6.
