Fig. 2: Loss of H3.3 in muscle gene regulatory regions in Hira-deficient cells. | Nature Communications

Fig. 2: Loss of H3.3 in muscle gene regulatory regions in Hira-deficient cells.

From: HIRA stabilizes skeletal muscle lineage identity

Fig. 2

a Total number of H3.3 called peaks (tag number) (q- value = 5e−2) in the ChIP-seq of C2C12 (gray, 38439 peaks) (n = 1) and Hira-KO cells (black, 3744 peaks) (n = 1). b Percentage of the total number of H3.3 peaks distributed in distinct genomic regions as indicated in C2C12 or Hira-KO cells. c, d ChIP-seq average signal profiles (ratio to input) in the promoter region (±3 kb around the TSS), TSS, gene body, and TES for H3.3 (c) and H3K27ac (d) in C2C12 (orange) and Hira KO (blue) shown for all genes, upregulated genes in Hira KO, downregulated in Hira-KO and skeletal muscle genes (GO:0007519). e, f ChIP-seq average signal profiles (ratio to input) of H3.3 (e) and H3K27ac (f) plotted on the control ATAC-seq peaks in C2C12 (orange) and Hira KO (blue). g–m ATAC-seq (green), ChIP-seq profiles for H3.3 (orange), and H3K27ac (gray) in the genomic loci of Pax7 (g), HeyL (h), Col5a1(i), Dmrt2 (j), Myod1 (k), Fgfr4 (l), and Eya4 (m).

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