Fig. 4: Hira-deficient satellite cells lose myogenic identity.

a Differential expression analysis of the RNA-seq analysis performed in 5 dpi satellite cells (n = 3 independent RNA samples for control and cKO). b MA-plot of control over Hira cKO satellite cells RNA-seq data. Significantly dysregulated genes are highlighted in red (FDR < 0.05). c, d Gene ontology analysis for biological processes of the downregulated (c) and upregulated (d) genes in cKO. Selected enriched terms are presented according to the fold enrichment. e Heatmap with the normalized reads per gene of control and Hira cKO RNA-seq data in individual triplicates. f RT-qPCR analyses in satellite cells (5 dpi) from Pax7^CreErt2;Hira^fl/+ and Pax7^CreErt2;Hira^fl/fl (n = 5 mice for Pax7, Myod1, Eya4, Angtp2; n = 4 mice for Myf5, Cdh5, Pdgfra; n = 3 mice for Fgfr4). For each gene, the mRNA levels of the control cells were normalized to 1. Error bars, mean ± SD, two-tailed paired t-test. g ChIP-RT-qPCR for H3.3 in Myod1 (−20 kb), Fgfr4 (+19.2 kb), Pax7 (+62.4 kb), Eya4 (−380 bp) and the negative control (−15 kb Myod1) of control (blue, n = 4) and Hira-depleted (red, n = 3) satellite cells (5 dpi). Error bars, mean ± SD, two-tailed unpaired t-test.