Fig. 1: Analysis of p32 expression levels in murine and human glioma samples.

a Confocal microscopy analysis of normal brain tissue (NBT), grade II diffuse astrocytoma, grade III anaplastic oligodendroglioma, and grade IV glioblastoma. Sections were stained with rabbit anti-p32 antibody. Negative control (Control) sections of GBM were incubated only with secondary Ab goat-anti-rabbit AlexaFluor488. The graph represents quantification of fluorescence intensity of p32 signal in normal brain and tumor sections from patients. Data represent mean ± SEM. Each dot represents the average of three images per sample. N = 6 (NBT), N = 4 (GII = Grade II), N = 5 (GIII = Grade III), and N = 5 (GIV = Grade IV) human samples per group. Statistical significance was determined using one-way ANOVA test with multiple comparisons adjustment. b Expression of p32 in murine 005 and patient-derived GBM83 xenograft tumors is confined just to the tumor area (GFP + area). Representative image of three independent mice per model. c p32 cell surface expression analyzed by FACS in murine glioma cells (005, AFFR53, O1), human cell lines (U87, U118, U178, U251) and patient-derived mesenchymal (GBM83, GBM1005, GBM1027) and proneural (GBM1079, GBM1051) GSCs. d FACS analysis of surface and intracellular expression levels of p32 in primary murine astrocytes, NIH3T3 fibroblasts and murine 005 and AFFR53 glioma cells (upper panels). Same analysis was performed with primary human astrocytes and skin fibroblasts, U87 and GBM83 human glioma cells (lower panels, light blue background). All histograms shown are representative of independent stainings for each cell line, N = 3 per cell line. All source data are provided as a Source Data file.